DA22780582 · publicată 08.04.2019 · finalizată 08.04.2019 · de la Dexter Com S.R.L. (Bucuresti)
T4 DNA Ligase - Producator Promega - kit 500units Weiss T4 DNA Ligase 10X Buffer C126B 1 × 200μl T4 DNA Ligase (HC) M179A 1 × 500u Storage Buffer: 10mM Tris-HCl (pH 7.4 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA and 50% glycerol. Source: Recombinant E. coli strain. QC Tests: Activity, SDS-PAGE/purity, DNase, endonuclease, RNase, blue/white cloning assay. Unit Definition: 0.01 Weiss unit of T4 DNA Ligase is the amount of enzyme required to catalyze the ligation of greater than 95% of 1μg of λ/HindIII fragments at 16°C in 20 minute